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KMID : 0545120070170010130
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 1 p.130 ~ p.137
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Ectopic Expression of Apple MbR7 Gene Induced Enhanced Resistance to Transgenic Arabidopsis Plant Against a Virulent Pathogen
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Lee Soo-Yeon
Choi Yeon-Ju
Ha Young-Mie
Lee Dong-Hee
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Abstract
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A disease resistance related gene, MbR7, was identified in the wild apple species, Malus baccata. The MbR7 gene has a single open reading frame (ORF) of 3,288 nucleotides potentially encoding a 1,095-amino acid protein. Its deduced amino acid sequence resembles the N protein of tobacco and the NL27 gene of potato and has several motifs characteristic of a TIR-NBS-LRR R gene subclass. Ectopic expression of MbR7 in Arabidopsis enhanced the resistance against a virulent pathogen, Pseudomonas syringae pv. tomato DC3000. Microarray analysis confirmed the induction of defense-related gene expression in 35S::MbR7 heterologous Arabidopsis plants, indicating that the MbR7 gene likely activates a downstream resistance pathway without interaction with pathogens. Our results suggest that MbR7 can be a potential target gene in developing a new disease-resistant apple variety
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KEYWORD
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Disease resistance gene, apple, TIR-NBS-LRR (Toll Interleukin 1 Receptor-Nucleotide Binding Site- Leucine Rich Repeat), ectopic expression
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